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Worked in a monkey lab?



“Buddy Capuano is new head vet”
Centerline Spring/Summer 2005

Saverio "Buddy" Capuano began March 1 as the WNPRC's attending veterinarian and associate director for the Animal Services Division. Capuano joins us from the University of Pittsburgh, where he served as a research assistant professor and attending veterinarian of the Magee-Womens Research Institute.

Tuberculosis, SIV/HIV, and herpesvirus are topics covered in Capuano’s widely published body of research. He presently collaborates on NIH grants supporting several nonhuman primate studies. Most recently he has worked on experiments refining assisted reproductive techniques and cloning in rhesus macaques and the use of the cynomolgus macaque as a model of Mycobacterium tuberculosis infection.
"I am looking forward to the challenge of heading the animal services division at the Primate Center," Capuano said. "I hope to work with everyone here to establish a nonhuman primate medicine residency at the Center. I am also excited about our impending growth and renovation."

There is something grotesque in the fact that the person hired by WPRC to care for the monkeys has a long history of making monkeys sick and then killing them.

Here is a brief history of Buddy's work prior to becoming the WPRC monkeys' primary medical care giver.

The 12 adult rhesus macaques (Macaca mulatta) used in this study were negative for SIV, simian retrovirus (type D), and simian T-lymphotropic viruses -1, -2, and –3… Briefly, all animals were inoculated intravenously (I.V.) and sacrificed either 2 weeks PI during the acute phase of infection or on progression to AIDS.

Restricted SIV replication in rhesus macaque lung tissues during the acute phase of infection. Fuller CL, Choi YK, Fallert BA, Capuano S 3rd, Rajakumar P, Murphey-Corb M, Reinhart TA. Am J Pathol. 2002 Sep;161(3):969-78.

The studies were done with the approval of the University of Pittsburgh Institutional Animal Care and Use Committee and included 15 adult rhesus macaques (Macaca mulatta) negative for SIV, simian retrovirus (type D), and simian T-lymphotropic viruses 1, 2, and 3. Eight macaques were inoculated intravenously with 1 mL of a 5×104 dilution of a cryopreserved stock of the SIV/B670 primary isolate, which was equivalent to 5 median tissue culture infectious doses. Four macaques (M5299, M5499, M5599, and M5699) were killed 2 weeks after infection (PI), but the infection had disseminated in only 3. The 4 remaining macaques were reinoculated with 1 mL of a 1:5 dilution of virus 1 week after the first inoculation to ensure infection and were killed on progression to AIDS. Four macaques (M0999, M5899, M5999, and M6299) were inoculated with the higher dose and killed 2 weeks PI for control purposes. M9597 was a long-term nonprogressor (LTNP) inoculated intravenously with bacille Calmette-Guérin 304 weeks after SIV/B670 infection and killed 80 weeks later as described previously.

Increased expression of the inflammatory chemokine CXC chemokine ligand 9/monokine induced by interferon-gamma in lymphoid tissues of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome. Reinhart TA, Fallert BA, Pfeifer ME, Sanghavi S, Capuano S 3rd, Rajakumar P, Murphey-Corb M, Day R, Fuller CL, Schaefer TM. Blood. 2002 May 1;99(9):3119-28.

Adult rhesus macaques (Macaca mulatta) of both sexes were used. Monkeys were infected as part of other studies with SIV/DeltaB670, a primary SIV isolate.24 Twelve animals infected with SIV were killed at 2 weeks after infection and categorized as having acute SIV infection. Seven SIV-infected animals were allowed to progress to AIDS. Seven animals were used as SIV naive controls. Animals were housed and all in vivo experiments were performed at the University of Pittsburgh Primate Facility for Infectious Disease Research using protocols approved by the institutional animal care and use committee.

Disrupted homeostasis of Langerhans cells and interdigitating dendritic cells in monkeys with AIDS. Zimmer MI, Larregina AT, Castillo CM, Capuano S 3rd, Falo LD Jr, Murphey-Corb M, Reinhart TA, Barratt-Boyes SM. Blood. 2002 Apr 15;99(8):2859-68.

Rhesus macaques (Macaca mulatta) were housed and maintained according to strict American Association of Laboratory Animal Care standards. Six rhesus macaques derived from vaccine trials, challenged with viral swarm SIVdeltaB670, and sacrificed were used in this study. Two macaques were involved in vaccine studies. Two macaques were administered PMPA 24 hours before inoculation with SIV. Macaques were infected intravenously with SIV/deltaB670 (n = 4), via bronchoscope with bronchial alveolar lavage from an animal infected with SIV/dB670 (n = 1), or rectally with SIV/dB670 and a subsequent infection via bronchoscope with bronchoalveolar lavage from an animal infected with SIV/dB670 (n = 1). Because the focus of this study was on CNS manifestations due to CNS SIV infection, the divergent routes of infection and clinical history in this group of animals does not impact directly on the final outcome of CNS disease. Ages of the macaques used in this study ranged from 33 to 100 months. Length of infection varied from 37 to 379 days. Macaques were sacrificed when moribund with AIDS (Table 2) . Only two macaques (macaques 603 and 221) exhibited neurological signs consisting of decreased feeding, decreased spontaneous movement, neglect of novel environmental stimuli, lethargic response to physical stimulation, and variable focal neurological signs. Two noninfected macaques served as controls. Complete necropsies were performed after humane sacrifice.

Macrophages relate presynaptic and postsynaptic damage in simian immunodeficiency virus encephalitis. Bissel SJ, Wang G, Ghosh M, Reinhart TA, Capuano S 3rd, Stefano Cole K, Murphey-Corb M, Piatak Jr M Jr, Lifson JD, Wiley CA. Am J Pathol. 2002 Mar;160(3):927-41.

What a caring and humane doctor.

Madison's Hidden Monkeys is a joint project of the
Alliance for Animals and the
Primate Freedom Project